Symposium Wageningen 07-06-2024

SVPW spring symposium, Friday, June 7th 2024 Hotel de Nieuwe Wereld, Marijkeweg 5, 6709 PE, Wageningen (NL)

We are happy to announce our upcoming spring symposium, for which the program can be found below. If you are interested to participate, please register before June 1st by using the “buy your ticket” button. The online registration fee is €30. This includes coffee, tea, lunch and closing drinks.

The registration fee at the door is €40, depending on availability.

The printed day program and abstracts will be available at the symposium.

We have eight confirmed speakers with interesting presentations related to a common theme:

Program:

9:30    Registration, coffee / tea & meet-up10:15   Opening by Prof. dr. Remko Offringa

10:20  Prof. dr. Sanne Abeln / Dr. Ronnie de JongeUtrecht University

AI in plant sciences

10:50   Dr. Hans Bethge – Leibniz Universität Hannover

Automated phenotyping in plant tissue culture

11:30  Iribov – Heerhugowaard

Automation in plant tissue culture

12:00  Viscon – ‘s-Gravendeel

Automation in plant tissue culture

12:30  Lunch & meet-up

13:45  SVPW info and questionnaire by Prof. dr. Remko Offringa

14:15  Prof. dr. ir. Joris SprakelLaboratory of Biochemistry, Wageningen University

Plants feel the Force: Green Mechanobiology

14:45   Ingo Nettersheim MSc.– Faculty of Aerospace Engineering, TU Delft

3D Printing Engineered Living Materials

15:15   Coffee / tea break

15:45  Dr. Lisa Van den Broeck – North Carolina State University / Protealis

3D bioprinting of plant cells.

16.15   Tom Clement MSc. – New Dawn Bio, Wageningen

The world’s biggest forest in a single cell

16:30   Closing drinks and meet-up

Summaries of the lectures on the SVPW spring symposium, Friday, June 7th, 2024)

Automated phenotyping in plant tissue culture
Dr. Hans Bethge – Leibniz Universität Hannover

Automated phenotyping of in vitro cultures can revolutionize trait evaluation by transitioning to continuous and objective quantification, as well as by enhancing accuracy, speed, and efficiency. Limited research exists on automated sensor usage in plant tissue culture, mainly focusing on “plant-to-sensor” approaches. Monitoring live aseptic cultures within closed vessels poses significant challenges, such as specular lighting at culture container materials and culture media surface and water condensation. This presentation will showcase a novel robotic phenotyping system using low-cost sensors to digitally quantify plant in vitro cultures. Various sensors, including RGB camera, laser distance sensor, micro spectrometer, and thermal camera, were deployed. The system quantified growth and detected physiological disorders using a convolutional neural network. Furthermore, strategies for integrating automated phenotyping in industrial micropropagation will be discussed.

Plants feel the Force: Green Mechanobiology
Prof. dr. ir. Joris Sprakel – Laboratory of Biochemistry, Wageningen University

Plant cells are continuously exposed to a wide diversity of mechanical stimuli, both from within (cells pushing and pulling on each other) and their environment (wind, pathogens trying to invade, etc.). These mechanical signals are perceived by plant cells and used as morphogenetic cues. Here I will give a brief overview of the work of my new group that tries to understand how this works by i) identifying the receptors of mechanical signals and their pathways, ii) making mechanical signals visible and measurable, and iii) exploring how mechanical signals can be engineered as a tool to control regeneration.

3D Printing Engineered Living Materials
Ir. Ingo Nettersheim - Faculty of Aerospace Engineering, Delft University of Technology

Natural structural materials like bird feathers and bone are formed with minimal energy input and are easily recycled by nature, which stands in stark contrast with most of the materials used in the present day. Despite the desire to replicate the materials found in Nature, recreating their intricate microstructure has been challenging with conventional manufacturing methods. We propose making these materials by using living cells. In this way, we can leverage their mechanisms to recreate nature’s microstructured materials. This talk will cover the fundamentals of 3D printing engineered living materials: how do you 3D print living cells, and how do you formulate an ink with optimal 3D-printing properties? Furthermore, we will explore recent work from our group printing microalgae and fungi, and discuss our ongoing work on 3D printing plant cells. Lastly, we will have a look at work from the field, which unveils the promising future of engineered living materials and their potential to revolutionize sustainable manufacturing.

3D bioprinting of plant cells
Dr. Lisa Van den Broeck – North Carolina State University / Protealis

Capturing cell-to-cell and cell-to-environment signals in a defined three-dimensional (3D) microenvironment is key to studying cellular functions, including cellular reprogramming towards tissue regeneration. A major challenge in current culturing methods is that these methods cannot accurately capture this multicellular 3D microenvironment. We have established the framework of 3D bioprinting with plant cells to study cell viability, cell division, and cell identity. To analyze the large image datasets generated during these long-term viability studies, using different tissues from Arabidopsis thaliana and Glycine max, we have developed an open-source image analysis pipeline, allowing us to high-throughput quantify both stained and unstained samples. The deposited Arabidopsis and soybean cells re-entered the cell cycle, which led to the formation of microcalli. Finally, we have shown that the identity of isolated cells of Arabidopsis roots expressing endodermal markers maintained for longer periods of time. We believe that the framework established in this study paves the way for the general use of 3D bioprinting for studying cellular reprogramming and cell cycle re-entry towards tissue regeneration.

The world’s biggest forest in a single cell
Tom Clement MSc., New Dawn Bio, Wageningen

With more tree species ending up on protected species lists and the global demand for wood increasing, finding sustainable ways to utilize this valuable renewable resource is essential. In this presentation, we explore the pressing challenges in modern forestry and discuss how plant cell culture is uniquely suited to solve them.

We introduce our bold mission of eliminating the need for traditional tree harvesting by growing wood directly from cells, show why wood is a great candidate for cell-based alternatives, and why now is the perfect time for cell-based wood.