Revolution in Plant Regeneration: The Power of Natural Auxins Enhanced

Deze blog is gebaseerd op de publicatie: “Transient efflux inhibition improves plant regeneration by natural auxins” from Omid Karami and Remko Offringa published on 15 Februari 2024 in The Plant Journal.

Plant genome editing and propagation are important tools in crop breeding and production. Both rely heavily on the development of efficient in vitro plant regeneration systems. Two prominent regeneration systems that are widely employed in crop production are somatic embryogenesis (SE) and de novo shoot regeneration. In many of the protocols for SE or shoot regeneration, explants are treated with the synthetic auxin analog 2,4-dichlorophenoxyacetic acid (2,4-D), since natural auxins, such as indole-3-acetic acid (IAA) or 4-chloroindole-3-acetic acid (4-Cl-IAA), are less effective or even fail to induce regeneration. The authors of the publication now show that natural auxins become efficient inducers of plant regeneration when their transport is transiently inhibited. Interestingly, a transport-inhibited natural auxin not only improves the regeneration process, but also leads to a more synchronized and homogenous development of the regenerated plants.

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