Category Archives: English

Deze blog is gebaseerd op de publicatie: "Transient efflux inhibition improves plant regeneration by natural auxins" from Omid Karami and Remko Offringa published on 15 Februari 2024 in The Plant Journal.


Plant genome editing and propagation are important tools in crop breeding and production. Both rely heavily on the development of efficient in vitro plant regeneration systems. Two prominent regeneration systems that are widely employed in crop production are somatic embryogenesis (SE) and de novo shoot regeneration. In many of the protocols for SE or shoot regeneration, explants are treated with the synthetic auxin analog 2,4-dichlorophenoxyacetic acid (2,4-D), since natural auxins, such as indole-3-acetic acid (IAA) or 4-chloroindole-3-acetic acid (4-Cl-IAA), are less effective or even fail to induce regeneration. The authors of the publication now show that natural auxins become efficient inducers of plant regeneration when their transport is transiently inhibited. Interestingly, a transport-inhibited natural auxin not only improves the regeneration process, but also leads to a more synchronized and homogenous development of the regenerated plants.

Author: prof.dr. D (Dolf) Weijers

You probably don't realise it when you walk on grass: the blades of grass not only sense the pressure of your feet, but adjust their growth accordingly. But how do plants register forces without a brain and central nervous system? How that works is what the GreenTE (Green Tissue Engineering) consortium is going to research over the next 10 years. The group has been awarded a €22.8 million Gravitation grant for this purpose. The consortium is led by Prof. Dolf Weijers and Prof. Joris Sprakel of the Laboratory of Biochemistry at Wageningen University & Research.